Purity Assessment of Drug Discovery Compound Libraries Using an Agilent Single Quadrupole LC/MS System Coupled to Diode Array and Evaporative Light Scattering Detectors
نویسنده
چکیده
Medicinal and analytical scientists supporting pharmaceutical medicinal chemistry efforts usually assess the purity of synthesized compounds prior to selecting them for biological activity. This application note demonstrates a fast and automated purity assessment of a 30 compound library using one generic method and a multidetector system. The use of the Agilent 6130 Series Single Quadrupole LC/MS System coupled to both an ultraviolet (UV) and an evaporative light scattering detector (ELSD), enables the detection of impurity peaks that may be missed by using only one mode of detection. Agilent Analytical Studio Reviewer software provides a simplifi ed user data processing interface for compound confi rmation and purity assessment. Authors Dr. N.S. Kameswara Rao and Dr. Subhabrata Sen GVK Biosciences, Hyderabad, India Syed Salman Lateef Vinayak AK Agilent Technologies, Bangalore, India
منابع مشابه
Simultaneous determination of bioactive constituents in Danggui Buxue Tang for quality control by HPLC coupled with a diode array detector, an evaporative light scattering detector and mass spectrometry.
Danggui Buxue Tang (DBT), a classical traditional Chinese formula comprising Radix Angelicae Sinensis (RAS) and Radix Astragali (RA), has been widely used to treat menopausal irregularity in Chinese women for nearly 800 years. In this study, a comprehensive analytical method of simultaneously determining the main types of bioactive constituents, eighteen in all from the formula, involving flavo...
متن کاملPerformance Characteristics of the Agilent 1290 Infinity Evaporative Light Scattering Detector
The Agilent 1290 Infinity Evaporative Light Scattering Detector (ELSD) provides a wide detection range for a variety of compounds with excellent sensitivity, down to low-nanogram levels, better efficiency and greater reproducibility. It is perfect for the analysis of semivolatile and nonvolatile compounds and compounds without UV chromophores such as most amino acids and sugars. The 1290 Infini...
متن کاملEnhancing the Productivity of Pharmaceutical Workfl ows Using the Agilent 6150 Single Quadrupole Mass Spectrometer
Simple, generic methods are commonly used for the routine screening of new molecular entities in a drug discovery environment. Here we describe a versatile LC/MS method that was developed using an Agilent 6150 Single Quadrupole (SQ) Mass Spectrometer coupled to an Agilent 1290 Infi nity LC System that is suitable for molecular weight confi rmation or impurity analysis. The UHPLC system is capab...
متن کاملHigh-Resolution Sampling 2D-LC Analysis of Mangosteen Pericarp Xanthones Using the Agilent 1290 Infinity II 2D-LC Solution
A high-resolution UHPLC analysis of xanthones present in the extracts of the pericarp of mangosteen (Garcinia mangostana L.) was carried out on an Agilent 1290 Infinity II LC. Excellent resolution and peak capacity (300 calculated at half height) were obtained within a total analysis time, including column reconditioning, of 16.5 minutes. The main xanthone (α-mangostin) was quantified in two ex...
متن کاملDetermination of Sweeteners, Preservatives, and Caffeine in Various Food and Consumer Products Using the Agilent 1290 Infi nity II LC
The Agilent 1290 Infi nity II LC was used to analyze additives such as sweeteners, preservatives, and caffeine in various food products, beverages, and consumer toothpaste. The developed method facilitates accurate and sensitive determination of nine additives using a diode array detector (DAD) and an evaporative light scattering detector (ELSD) placed in series. After the DAD, a valve was inst...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013